Studies on Bile Pigments. III. Separation of Natural Bilirubins
نویسندگان
چکیده
منابع مشابه
Lv. Studies on Bile Pigments
THE study of bile pigment derivatives is important because these substances represent the end-products of haemoglobin metabolism. The destruction of haemoglobin both normal and pathological can thus be measured by quantitative determination of the urobilin and urobilinogen in urine and faeces. On the other hand, when pathological destruction of haemoglobin can be excluded, urobilinor urobilinog...
متن کاملOrigin of Bile Pigments
S. H. ROBINSON (Boston) described a new experimental technique for studying the early labelled component of bilirubin in the ligated bile duct of the Gunn rat. Using 14C bilirubin he measured the total miscible bilirubin pool. In addition, the rate of bile pigment formation following the administration of 2-14C glycine was estimated. This allowed precise assessment of bilirubin and haem product...
متن کاملStudies on the determination of bile pigments. III. Standardization of the determination of urobilinogen as urobilinogen-aldehyde.
Several preparations of urobilin and stercobilin were investigated with regard to their value as standards for the determination of urobilinogen and urobilin in biologic specimens. Products of commercial origin were found to be impure and therefore unsuitable for this purpose. Absorptivities were measured for solutions of the pigments in 3% FICI in methanol and for the chromogens, obtained by f...
متن کاملCxlvii. Studies on Bile Pigments. Iii. the Quantitative Determination of Urobilin and Urobilinogen in Urine and Faeces
IN a previous paper [Naumann, 1936] the qualitative tests for urobilinogen and urobilin have been studied. In the following the quantitative aspect of the problem is treated. A basis for the estimation of urobilin and related compounds was first introduced by Terwen [1924; 1925]. The main features of this method consist in determining the sum of urobilinogen and urobilin as urobilinogen after r...
متن کاملStudies on bile acids: The microquantitative separation of cellular bile acids by gas-liquid chromatography.
1. A method is described for the quantitative isolation of bile acids from cellular material. Homogenates of rat liver are freeze-dried and extracted exhaustively with 95% (v/v) ethanol containing 0.1% (v/v) of aq. ammonia (sp.gr. 0.88) and purified by anion-exchange chromatography on Amberlyst A-26. 2. The extracted bile acid conjugates are subjected to either of two hydrolytic procedures, one...
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ژورنال
عنوان ژورنال: Proceedings of the Japan Academy
سال: 1956
ISSN: 0021-4280
DOI: 10.2183/pjab1945.32.306